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This system has high specificity, so the likelihood of other genes being affected by the zinc finger protein transcription factor is low. The expression of the zinc finger protein transcription factor can also be made inducible, so that it is possible to control when the gene of interest is activated. The authors targeted the human parathyroid hormone receptor 1 (PTHR1), which is a G protein-coupled receptor that has a role in calcium homeostasis and bone metabolism. A zinc finger protein transcription factor that targets PTHR1 was identified, and used to induce its expression in HEK293 cells, which do not normally express PTHR1. When the zinc finger protein transcription factor was induced, the HEK293 cells began to express PTHR1. The activity of the expressed PTHR1 was confirmed by increase in the levels of cAMP produced by the cells. The authors then used known PTHR1 ligands, and this led to the reduction in cAMP levels, thus showing that its function was reduced. The authors concluded that this approach can be used to study effects of potential therapeutic compounds on target genes.
1 - 20 of 472 Works in Mischa Lecter Pages Navigation Listing Works Tags Summary In the 16th Century, after witnessing Micha´s murder, Hannibal goes after Grutas Gang members. He manage to capture the youngest member of the group and the one presumably hold responsible for his sister death, Will Graham. Filled with rage, Hannibal keeps him prisoner and tries to break him into submission. Language: English Words: 88, 573 Chapters: 16 /? Kudos: 198 Bookmarks: 15 Hits: 6399 "Modern romance, like Greek tragedy, celebrates the mystery of dismemberment, which is life in time. The happy ending is justly scorned as a misrepresentation; for the world, as we know it, as we have seen it, yeilds but one ending: death, disintegration, dismemberment, and the crucifixion of our heart with the passing of the forms that we have loved. " ~ Joseph Campbell, The Hero With A Thousand Faces Series Part 2 of MONSTER. 721 1/? 3 46 Gentle. That was the word the monster had used. Will would hold onto that word as if it were the only thing in the universe.